Numb Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27181

Novus Biologicals, part of Bio-Techne
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NBP2-27181SS
NBP2-27181
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Primate

Cited:

Rat

Predicted:

Monkey (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Numb Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 12-40 of human NUMB was used as the immunogen for the antibody, GenBank no gb|AAH20788.1|.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Numb Antibody

Western Blot: Numb Antibody [NBP2-27181]

Western Blot: Numb Antibody [NBP2-27181]

Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test
Immunohistochemistry-Paraffin: Numb Antibody [NBP2-27181]

Immunohistochemistry-Paraffin: Numb Antibody [NBP2-27181]

Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human adrenal tissue stained with NUMB antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: Numb Antibody [NBP2-27181]

Immunohistochemistry-Paraffin: Numb Antibody [NBP2-27181]

Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human spleen stained with NUMB antibody at 10 ug/ml.

Applications for Numb Antibody

Application
Recommended Usage

Immunohistochemistry

reported in scientific literature (PMID 30256757)

Immunohistochemistry-Paraffin

5-10ug/ml~

Western Blot

1-2ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Numb

Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.

Alternate Names

m-numb, mnb, S171

Gene Symbol

NUMB

UniProt

P49757 (Human)

Additional Numb Products

Product Documents for Numb Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Numb Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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