Nup153 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93329

Novus Biologicals, part of Bio-Techne
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NB100-93329
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Nup153 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human nucleoporin 153kDa using the numbering given in entry NP_005115.2 (GeneID 9972).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nup153 Antibody

Western Blot: Nup153 Antibody [NB100-93329]

Western Blot: Nup153 Antibody [NB100-93329]

Western Blot: Nup153 Antibody [NB100-93329] - Knockdown of NUP153 in MDA-MB-231 breast cancer cells. Lane 1: ladder. Lane 2: Scramble Control Lane 3: NUP153 knockdown. Substantially reduced protein detection in knockdown sample and not in scramble control reflect antibody's unique specificity for Nup153. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Nup153 Antibody [NB100-93329]

Immunocytochemistry/ Immunofluorescence: Nup153 Antibody [NB100-93329]

Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NB100-93329] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross adsorbed Antibody used at a dilution of 1:100.
Western Blot: Nup153 Antibody [NB100-93329]

Western Blot: Nup153 Antibody [NB100-93329]

Western Blot: Nup153 Antibody [NB100-93329] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-9329. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-9330.

Applications for Nup153 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. Nup153 antibody validated for WB from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-93329 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nup153

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by this gene has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.

Alternate Names

CG4453, Dmel\CG4453, dmNup153, dNup153, HNUP153, N153, nuclear pore complex protein hnup153,153 kDa nucleoporin, nuclear pore complex protein Nup153, nucleoporin 153kD, nucleoporin 153kDa, Nucleoporin Nup153, Nup 153, NUP153, Nup153 Nucleoporin 153

Entrez Gene IDs

9972 (Human)

Gene Symbol

NUP153

UniProt

P49790 (Human)

Additional Nup153 Products

Product Documents for Nup153 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nup153 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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