Key Product Details

Species Reactivity

Validated:

Rat

Applications

Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # RL31

Concentration

0.5 mg/ml

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Product Summary for NUP62 Antibody (RL31)

Immunogen

O-linked glycoprotein-enriched fraction from rat liver nuclear envelopes raised in Armenian hamsters.

Localization

Central region of the nuclear pore. During mitotic cell division, it associates with the poles of the mitotic spindle.

Specificity

Detects the nucleoporin p62 O-linked glycoprotein in the p62 complex from rat tissues.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for NUP62 Antibody (RL31)

Western Blot: NUP62 Antibody (RL31) [NB120-2736]

Western Blot: NUP62 Antibody (RL31) [NB120-2736]

Western Blot: NUP62 Antibody (RL31) [NB120-2736] - Analysis of Nucleoporin p62 was performed by loading whole cell lysates (20ug) of the indicated samples and 5ul of prestained protein ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. Nucleoporin p62 was detected at ~62kD in samples using a Nucleoporin p62 (RL31) monoclonal antibody at a dilution of 1:200 overnight at 4C on a rocking platform, followed by a goat anti-Armenian hamster IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature.

Applications for NUP62 Antibody (RL31)

Application
Recommended Usage

Immunofluorescence

1:50

Western Blot

1:200
Application Notes
WB: Detects a single 62 kDa protein representing nucleoporin p62 in rat liver nuclear envelope fractions. IF: Staining of p62 in rat liver with this antibody results in staining of both the nucleoplasmic and cytoplasmic surfaces of the nuclear pore complex (NPC).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2-7.4) with 1mg/ml BSA and 30% glycerol

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NUP62

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Alternate Names

DKFZp547L134,62 kDa nucleoporin, FLJ20822, FLJ43869, IBSNp62, MGC841, nuclear pore glycoprotein p62, nucleoporin 62kD, nucleoporin 62kDa, SNDINucleoporin Nup62

Entrez Gene IDs

65274 (Rat)

Gene Symbol

NUP62

UniProt

P17955 (Rat)

Additional NUP62 Products

Product Documents for NUP62 Antibody (RL31)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NUP62 Antibody (RL31)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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