NXT1 Antibody (4F11)

Novus Biologicals, part of Bio-Techne | Catalog # H00029107-M08

Novus Biologicals, part of Bio-Techne
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H00029107-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 4F11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NXT1 Antibodies »

Product Specifications

Immunogen

NXT1 (AAH00759, 1 a.a. ~ 140 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASVDFKTYVDQACRAAEEFVNVYYTTMDKRRRLLSRLYMGTATLVWNGNAVSGQESSSEFFEMLPSSEFQISVVDCQPVHDEATPSQTTVLVVICGSVKFEGNKQRDFNQNFILTAQASPSNTVWKIASDCFRFQDWAS

Reactivity Notes

Human. Other species not tested.

Specificity

NXT1 - NTF2-like export factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Scientific Data Images for NXT1 Antibody (4F11)

Western Blot: NXT1 Antibody (4F11) [H00029107-M08]

Western Blot: NXT1 Antibody (4F11) [H00029107-M08]

Western Blot: NXT1 Antibody (4F11) [H00029107-M08] - Analysis of NXT1 expression in transfected 293T cell line by NXT1 monoclonal antibody (M08), clone 4F11. Lane 1: NXT1 transfected lysatE (15.51 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NXT1 Antibody (4F11) [H00029107-M08]

Immunocytochemistry/ Immunofluorescence: NXT1 Antibody (4F11) [H00029107-M08]

Immunocytochemistry/Immunofluorescence: NXT1 Antibody (4F11) [H00029107-M08] - Analysis of monoclonal antibody to NXT1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: NXT1 Antibody (4F11) [H00029107-M08]

Western Blot: NXT1 Antibody (4F11) [H00029107-M08]

Western Blot: NXT1 Antibody (4F11) [H00029107-M08] - Western blot analysis of NXT1 over-expressed 293 cell line, cotransfected with NXT1 Validated Chimera RNAi or non-transfected control. Blot probed with H00029107-M08. GAPDH (36.1 kDa) used as loading control.
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Applications for NXT1 Antibody (4F11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NXT1

The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene.

Alternate Names

MTR2NTF2-related export protein 1, NTF2-like export factor 1, NTX2-like export factor1, NUTF-like export factor 1, P15, Protein p15

Entrez Gene IDs

29107 (Human)

Gene Symbol

NXT1

OMIM

605811 (Human)

UniProt

AAH00759 (Human)

Additional NXT1 Products

Product Documents for NXT1 Antibody (4F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NXT1 Antibody (4F11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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