O-GlcNAc Antibody (RL2) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB300-524B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB300-524AF350

Alexa Fluor 405

NB300-524AF405

Alexa Fluor 488

NB300-524AF488

Alexa Fluor 532

NB300-524AF532

Alexa Fluor 594

NB300-524AF594

Alexa Fluor 647

NB300-524AF647

Alexa Fluor 700

NB300-524AF700

Alexa Fluor 750

NB300-524AF750

Allophycocyanin

NB300-524APC

Allophycocyanin/Cy7

NB300-524APCCY7

CoraFluor 1

NB300-524CL1

DyLight 350

NB300-524UV

DyLight 405

NB300-524V

DyLight 488

NB300-524G

DyLight 550

NB300-524R

DyLight 594

NB300-524DL594

DyLight 650

NB300-524C

DyLight 680

NB300-524FR

DyLight 755

NB300-524IR

FITC

NB300-524F

HRP

NB300-524H

Janelia Fluor 525

NB300-524JF525

Janelia Fluor 549

NB300-524JF549

Janelia Fluor 646

NB300-524JF646

Janelia Fluor 669

NB300-524JF669

mFluor Violet 450 SE

NB300-524MFV450

mFluor Violet 500 SE

NB300-524MFV500

mFluor Violet 610 SE

NB300-524MFV610

PE

NB300-524PE

PE/Atto594

NB300-524PEATT594

PE/Cy5.5

NB300-524PECY55

PE/Cy7

NB300-524PECY7

Forumulation

Catalog #

BSA Free

NB300-524

Azide and BSA Free

NBP2-80892

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Drosophila, Fish, Hamster, Primate, Virus, Xenopus

Applications

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # RL2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all O-GlcNAc Antibodies »

Product Specifications

Immunogen

Pore complex-lamina fraction purified from rat liver nuclear envelopes.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 26004176). Xenopus reactivity reported in scientific literature (PMID: 17329255). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic and Nuclear

Specificity

Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for O-GlcNAc Antibody (RL2) [Biotin]

Application

Recommended Usage

Chromatin Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: O-GlcNAc

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Alternate Names

GlcNAc, O-linked N-acetylglucosamine

Additional O-GlcNAc Products

Product Documents for O-GlcNAc Antibody (RL2) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for O-GlcNAc Antibody (RL2) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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