O-GlcNAc Antibody (RL2) [Alexa Fluor® 647]
Novus Biologicals, part of Bio-Techne | Catalog # NB300-524AF647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Drosophila, Fish, Hamster, Primate, Virus, Xenopus
Cited:
Mouse
Applications
Validated:
Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # RL2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID: 26004176). Xenopus reactivity reported in scientific literature (PMID: 17329255). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cytoplasmic and Nuclear
Specificity
Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for O-GlcNAc Antibody (RL2) [Alexa Fluor® 647]
Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on RH30 cells with O-GlcNAc [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor (R) 647] [NB300-524AF647] - An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on SK-MEL-28 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Alexa Fluor 647.
Applications for O-GlcNAc Antibody (RL2) [Alexa Fluor® 647]
Application
Recommended Usage
Chromatin Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB300-524AF647 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: O-GlcNAc
Alternate Names
GlcNAc, O-linked N-acetylglucosamine
Additional O-GlcNAc Products
Product Documents for O-GlcNAc Antibody (RL2) [Alexa Fluor® 647]
Product Specific Notices for O-GlcNAc Antibody (RL2) [Alexa Fluor® 647]
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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