O-GlcNAc Transferase p110 subunit Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57845

Novus Biologicals, part of Bio-Techne
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NB100-57845
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all O-GlcNAc Transferase/OGT Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1; NP_858059.1.

Epitope

YEHPKDLKLSDGR

Specificity

This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Rat pancreas lysate (35 ug protein in RIPA buffer). Primary antibody at 0.05 ug/mL, 1 hr incubation. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and membrane/cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Human cortex tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for O-GlcNAc Transferase p110 subunit Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.05 - 0.2 ug/mL
Application Notes
WB: Approx 110k Da band observed in pancreas lysates from rat (calculated MW of 117 kDa according to NP_858058.1. An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Entrez Gene IDs

8473 (Human); 108155 (Mouse); 26295 (Rat)

Gene Symbol

OGT

UniProt

NP_858058.1 (Human)

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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