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OBFC1 Antibody (OTI2E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01006

Novus Biologicals, part of Bio-Techne
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NBP2-01006
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot

Cited:

Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E4

Concentration

0.4 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human OBFC1 (NP_079204) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OBFC1 Antibody (OTI2E4)

Western Blot: OBFC1 Antibody (OTI2E4) [NBP2-01006]

Western Blot: OBFC1 Antibody (OTI2E4) [NBP2-01006]

Western Blot: OBFC1 Antibody (2E4) [NBP2-01006] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OBFC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OBFC1.
Immunocytochemistry/Immunofluorescence: OBFC1 Antibody (2E4) [NBP2-01006] Staining of COS7 cells transiently transfected by pCMV6-ENTRY OBFC1.
Flow Cytometry: OBFC1 Antibody (OTI2E4) [NBP2-01006]

Flow Cytometry: OBFC1 Antibody (OTI2E4) [NBP2-01006]

Flow Cytometry: OBFC1 Antibody (2E4) [NBP2-01006] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OBFC1 antibody, and then analyzed by flow cytometry.

Applications for OBFC1 Antibody (OTI2E4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Proximity Ligation Assay

Reported in scientific literature (PMID:34339741)

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OBFC1

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM].

Alternate Names

AAF44, AAF-44, alpha accessory factor 44, bA541N10.2, CST complex subunit STN1, oligonucleotide/oligosaccharide-binding fold containing 1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, replication protein A 32 kDa subunit, RP11-541N10.2, RPA-32, STN1FLJ22559, Suppressor of cdc thirteen homolog

Entrez Gene IDs

79991 (Human)

Gene Symbol

STN1

Additional OBFC1 Products

Product Documents for OBFC1 Antibody (OTI2E4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OBFC1 Antibody (OTI2E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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