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OBFC1 Antibody (OTI2E4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73138

Novus Biologicals, part of Bio-Techne
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NBP2-73138
Conjugate
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human OBFC1 (NP_079204) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OBFC1 Antibody (OTI2E4) - Azide and BSA Free

Western Blot: OBFC1 Antibody (OTI2E4)Azide and BSA Free [NBP2-73138]

Western Blot: OBFC1 Antibody (OTI2E4)Azide and BSA Free [NBP2-73138]

Western Blot: OBFC1 Antibody (OTI2E4) - Azide and BSA Free [NBP2-73138] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OBFC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OBFC1.
Immunocytochemistry/ Immunofluorescence: OBFC1 Antibody (OTI2E4) - Azide and BSA Free [NBP2-73138]

Immunocytochemistry/ Immunofluorescence: OBFC1 Antibody (OTI2E4) - Azide and BSA Free [NBP2-73138]

Immunocytochemistry/Immunofluorescence: OBFC1 Antibody (OTI2E4) - Azide and BSA Free [NBP2-73138] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY OBFC1.
Flow Cytometry: OBFC1 Antibody (OTI2E4) - Azide and BSA Free [NBP2-73138]

Flow Cytometry: OBFC1 Antibody (OTI2E4) - Azide and BSA Free [NBP2-73138]

Flow Cytometry: OBFC1 Antibody (OTI2E4) - Azide and BSA Free [NBP2-73138] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-OBFC1 antibody, and then analyzed by flow cytometry.

Applications for OBFC1 Antibody (OTI2E4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OBFC1

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNApolymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 (PubMed19119139)). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM613130) (Miyake et al., 2009 (PubMed 19854130)).(supplied by OMIM)

Alternate Names

AAF44, AAF-44, alpha accessory factor 44, bA541N10.2, CST complex subunit STN1, oligonucleotide/oligosaccharide-binding fold containing 1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, replication protein A 32 kDa subunit, RP11-541N10.2, RPA-32, STN1FLJ22559, Suppressor of cdc thirteen homolog

Gene Symbol

STN1

Additional OBFC1 Products

Product Documents for OBFC1 Antibody (OTI2E4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OBFC1 Antibody (OTI2E4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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