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OBSCN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88818

Novus Biologicals, part of Bio-Techne
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NBP1-88818
NBP1-88818-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for OBSCN Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ACVVVSGSESEAESSSGGELDDAFRRAARRLHRLFRTKSPAEVSDEELFLSADEGPAEPEEPADWQTYREDEHFICIRFEALTEARQAVTRFQEMFATLGIGVEIKLVEQGPRRVEMCISKETPAPVVPPEPLPSLLTSDAAP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OBSCN Antibody

Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818]

Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818]

Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Analysis in human skeletal muscle and liver tissues. Corresponding OBSCN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: OBSCN Antibody [NBP1-88818]

Immunocytochemistry/ Immunofluorescence: OBSCN Antibody [NBP1-88818]

Immunocytochemistry/Immunofluorescence: OBSCN Antibody [NBP1-88818] - Staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818]

Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818]

Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.

Applications for OBSCN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OBSCN

OBSCN - obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Alternate Names

ARHGEF30, DKFZp666E245, EC 2.7.11.1, FLJ14124, KIAA1556MGC120412, KIAA1639MGC138590, MGC120409, MGC120410, MGC120411, obscurin, obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF, obscurin, myosin light chain kinase, Obscurin-MLCK, Obscurin-myosin light chain kinase, Obscurin-RhoGEF, UNC89

Gene Symbol

OBSCN

Additional OBSCN Products

Product Documents for OBSCN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OBSCN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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