Occludin Antibody (1G7)

Novus Biologicals, part of Bio-Techne | Catalog # H00004950-M01

Novus Biologicals, part of Bio-Techne
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H00004950-M01
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Occludin Antibodies »

Product Specifications

Immunogen

OCLN (NP_002529, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

Specificity

OCLN - occludin

Marker

Tight Junctions Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Occludin Antibody (1G7)

Western Blot: Occludin Antibody (1G7) [H00004950-M01]

Western Blot: Occludin Antibody (1G7) [H00004950-M01]

Western Blot: Occludin Antibody (1G7) [H00004950-M01] - OCLN monoclonal antibody (M01), clone 1G7 Analysis of OCLN expression in HL-60.
Immunocytochemistry/ Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01]

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01]

Immunocytochemistry/Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01] - Analysis of monoclonal antibody to OCLN on HeLa cell. Antibody concentration 10 ug/ml.
Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01]

Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01]

Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01] - Analysis of OCLN transfected lysate using anti-OCLN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OCLN MaxPab rabbit polyclonal antibody.
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Applications for Occludin Antibody (1G7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Occludin

This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.

Alternate Names

BLCPMG, OCLN

Entrez Gene IDs

4950 (Human)

Gene Symbol

OCLN

OMIM

602876 (Human)

UniProt

NP_002529 (Human)

Additional Occludin Products

Product Documents for Occludin Antibody (1G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Occludin Antibody (1G7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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