Occludin Antibody (1G7)
Novus Biologicals, part of Bio-Techne | Catalog # H00004950-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1G7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
OCLN (NP_002529, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
Specificity
OCLN - occludin
Marker
Tight Junctions Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Occludin Antibody (1G7)
Western Blot: Occludin Antibody (1G7) [H00004950-M01]
Western Blot: Occludin Antibody (1G7) [H00004950-M01] - OCLN monoclonal antibody (M01), clone 1G7 Analysis of OCLN expression in HL-60.Immunocytochemistry/ Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01]
Immunocytochemistry/Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01] - Analysis of monoclonal antibody to OCLN on HeLa cell. Antibody concentration 10 ug/ml.Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01]
Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01] - Analysis of OCLN transfected lysate using anti-OCLN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OCLN MaxPab rabbit polyclonal antibody.Applications for Occludin Antibody (1G7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Occludin
Alternate Names
BLCPMG, OCLN
Entrez Gene IDs
4950 (Human)
Gene Symbol
OCLN
OMIM
602876 (Human)
UniProt
Additional Occludin Products
Product Documents for Occludin Antibody (1G7)
Product Specific Notices for Occludin Antibody (1G7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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