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Occludin Antibody (CL1567) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43903

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL1567

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q16625, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Occludin Antibody (CL1567) - Azide and BSA Free

Occludin Antibody (CL1567) - Azide and BSA Free Western Blot: Occludin Antibody [NBP3-43903]

Western Blot: Occludin Antibody [NBP3-43903]

Lane 1: Marker [kDa]
Lane 2: Human cell line CACO-2
Lane 3: Human cell line U-87
Occludin Antibody (CL1567) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43903]

Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43903]

Staining of human breast cancer shows membranous immunoreactivity in tumor cells.
Occludin Antibody (CL1567) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP3-43903]

Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP3-43903]

Staining of CACO-2 cells using the Anti-Occludin monoclonal antibody) .

Applications for Occludin Antibody (CL1567) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

Product Documents for Occludin Antibody (CL1567) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Occludin Antibody (CL1567) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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