Occludin Antibody (CL1608)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52973
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Immunohistochemistry-Paraffin-NBP2-52973-img0001.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL1608
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEED
Epitope
RYSSGGNFETPSKRA
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%). Use in Mouse reported in scientific publication (PMID: 32485864).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Occludin Antibody (CL1608)
![Western Blot: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Western-Blot-NBP2-52973-img0008.jpg "Western Blot: Occludin Antibody (CL1608) [NBP2-52973]")
Western Blot: Occludin Antibody (CL1608) [NBP2-52973]
Western Blot: Occludin Antibody (CL1608) [NBP2-52973] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)![Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Immunocytochemistry-Immunofluorescence-NBP2-52973-img0009.jpg "Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1608) [NBP2-52973]")
Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1608) [NBP2-52973]
Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1608) [NBP2-52973] - Staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Immunohistochemistry-Paraffin-NBP2-52973-img0007.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]")
Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]
Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973] - Staining of human tonsil shows membranous immunoreactivity in squamous epithelium cells.![Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Immunohistochemistry-Paraffin-NBP2-52973-img0002.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Immunohistochemistry-Paraffin-NBP2-52973-img0003.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Immunohistochemistry-Paraffin-NBP2-52973-img0004.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1608-Immunohistochemistry-Paraffin-NBP2-52973-img0005.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]")
Applications for Occludin Antibody (CL1608)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
IHC-P,Retrieval method: HIER pH6, ICC/IF,Fixation/Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Occludin
Alternate Names
BLCPMG, OCLN
Gene Symbol
OCLN
Additional Occludin Products
Product Documents for Occludin Antibody (CL1608)
Product Specific Notices for Occludin Antibody (CL1608)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...