OCRL Antibody (S166A-26)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42193
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S166A-26
Concentration
1 mg/ml
Product Summary for OCRL Antibody (S166A-26)
Immunogen
Fusion protein amino acids 1-901 (full-length) of human INPP5F. Rat: 93% identity (845/904 amino acids identical). Mouse: 91% identity (824/901 amino acids identical) ~50% identity with INPP5b.
Reactivity Notes
Immunogen displays sequence identity for non-tested species: Mouse and Rat.
Localization
Cytoplasmic Vesicle , Endosome , Endosome Membrane , Membrane , Golgi Apparatus
Specificity
Approx 50% identity with INPP5b.Detects approx 100kDa. Cross-reacts with INPP5b.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for OCRL Antibody (S166A-26)
![Western Blot: OCRL Antibody (S166A-26) [NBP2-42193]](https://resources.bio-techne.com/images/products/OCRL-Antibody-S166A-26-Western-Blot-NBP2-42193-img0001.jpg "Western Blot: OCRL Antibody (S166A-26) [NBP2-42193]")
Western Blot: OCRL Antibody (S166A-26) [NBP2-42193]
Western Blot: OCRL Antibody (S166A-26) [NBP2-42193] - analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of 100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 100 kDa.![Immunocytochemistry/ Immunofluorescence: OCRL Antibody (S166A-26) [NBP2-42193]](https://resources.bio-techne.com/images/products/OCRL-Antibody-S166A-26-Immunocytochemistry-Immunofluorescence-NBP2-42193-img0002.jpg "Immunocytochemistry/ Immunofluorescence: OCRL Antibody (S166A-26) [NBP2-42193]")
Immunocytochemistry/ Immunofluorescence: OCRL Antibody (S166A-26) [NBP2-42193]
Immunocytochemistry/Immunofluorescence: OCRL Antibody (S166A-26) [NBP2-42193] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Endosome, Endosome Membrane, Membrane, Golgi Apparatus, Nucleus. Magnification: 60X.Applications for OCRL Antibody (S166A-26)
Application
Recommended Usage
Western Blot
1:1000
Application Notes
1 ug/ml of INPP5F Antibody was sufficient for detection of INPP5F in 20 ug of COS cells transiently transformed with GFP-tagged OCLR lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OCRL
Alternate Names
EC 3.1.3.36, INPP5F, LOCR, Lowe oculocerebrorenal syndrome protein, NPHL2, OCRL1inositol polyphosphate 5-phosphatase OCRL-1, oculocerebrorenal syndrome of Lowe, phosphatidylinositol polyphosphate 5-phosphatase
Gene Symbol
OCRL
UniProt
Additional OCRL Products
Product Documents for OCRL Antibody (S166A-26)
Product Specific Notices for OCRL Antibody (S166A-26)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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