OCT1 Antibody (8L4Y5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16246
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8L4Y5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for OCT1 Antibody (8L4Y5)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human OCT1 (P14859). MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMT
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for OCT1 Antibody (8L4Y5)
![Western Blot: OCT1 Antibody (8L4Y5) [NBP3-16246]](https://resources.bio-techne.com/images/products/OCT1-Antibody-8L4Y5-Western-Blot-NBP3-16246-img0003.jpg "Western Blot: OCT1 Antibody (8L4Y5) [NBP3-16246]")
Western Blot: OCT1 Antibody (8L4Y5) [NBP3-16246]
Western Blot: OCT1 Antibody (8L4Y5) [NBP3-16246] - Western blot analysis of extracts of various cell lines, using POU2F1/OCT1 Rabbit mAb (NBP3-16246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.![Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246]](https://resources.bio-techne.com/images/products/OCT1-Antibody-8L4Y5-Immunohistochemistry-Paraffin-NBP3-16246-img0002.jpg "Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246]")
Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246]
Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246] - Immunohistochemistry of paraffin-embedded mouse liver using POU2F1/OCT1 Rabbit mAb (NBP3-16246) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246]](https://resources.bio-techne.com/images/products/OCT1-Antibody-8L4Y5-Immunohistochemistry-Paraffin-NBP3-16246-img0001.jpg "Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246]")
Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246]
Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246] - Immunohistochemistry of paraffin-embedded human esophageal cancer using POU2F1/OCT1 Rabbit mAb (NBP3-16246) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for OCT1 Antibody (8L4Y5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: OCT1
Long Name
Octamer-binding Transcription Factor 1
Alternate Names
FLJ42836, NF-A1, Oct-1, OCT1class 2, transcription factor 1, OTF1, POU class 2 homeobox 1, POU domain class 2, transcription factor 1, POU2F1
Gene Symbol
POU2F1
Additional OCT1 Products
Product Documents for OCT1 Antibody (8L4Y5)
Product Specific Notices for OCT1 Antibody (8L4Y5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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