OCT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05865
![Western Blot: OCT1 Antibody [NBP3-05865]](https://resources.bio-techne.com/images/products/OCT1-Antibody-Western-Blot-NBP3-05865-img0002.jpg "Western Blot: OCT1 Antibody [NBP3-05865]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for OCT1 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human OCT1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for OCT1 Antibody
Western Blot: OCT1 Antibody [NBP3-05865]
Western Blot: OCT1 Antibody [NBP3-05865] - Lane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:76 kDaObserved band size:76 kDa(We are unsure as to the identity of these extra bands.)![Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP3-05865]](https://resources.bio-techne.com/images/products/OCT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05865-img0001.jpg "Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP3-05865]")
Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP3-05865]
Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP3-05865] - Staining of POU2F1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human POU2F1 polyclonal antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.Applications for OCT1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OCT1
Long Name
Octamer-binding Transcription Factor 1
Alternate Names
FLJ42836, NF-A1, Oct-1, OCT1class 2, transcription factor 1, OTF1, POU class 2 homeobox 1, POU domain class 2, transcription factor 1, POU2F1
Gene Symbol
POU2F1
Additional OCT1 Products
Product Documents for OCT1 Antibody
Product Specific Notices for OCT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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