OCT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05865

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all OCT1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human OCT1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for OCT1 Antibody

Western Blot: OCT1 Antibody [NBP3-05865]

Western Blot: OCT1 Antibody [NBP3-05865]

Western Blot: OCT1 Antibody [NBP3-05865] - Lane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:76 kDaObserved band size:76 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP3-05865]

Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP3-05865]

Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP3-05865] - Staining of POU2F1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human POU2F1 polyclonal antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.

Applications for OCT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OCT1

Oct1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR and interacts with NR3C1, AR and PGR. Oct1 is Ubiquitous and belongs to the POU transcription factor family class 2 subfamily. Oct1 contains 1 homeobox domain.

Long Name

Octamer-binding Transcription Factor 1

Alternate Names

FLJ42836, NF-A1, Oct-1, OCT1class 2, transcription factor 1, OTF1, POU class 2 homeobox 1, POU domain class 2, transcription factor 1, POU2F1

Gene Symbol

POU2F1

Additional OCT1 Products

Product Documents for OCT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for OCT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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