OCT4 Antibody (735)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15051

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-15051
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Primate (100%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 735

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all OCT4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Cat (89%), Pig (88%), Rabbit (85%), Bovine (85%).

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OCT4 Antibody (735)

Western Blot: OCT4 Antibody (735) [NBP2-15051]

Western Blot: OCT4 Antibody (735) [NBP2-15051]

Western Blot: OCT4 Antibody (735) [NBP2-15051] - A. 30 ug human ESC whole cell lysate/extract 10 % SDS-PAGE dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (735) [NBP2-15051]

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (735) [NBP2-15051]

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (735) [NBP2-15051] - Human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Flow Cytometry: OCT4 Antibody (735) [NBP2-15051]

Flow Cytometry: OCT4 Antibody (735) [NBP2-15051]

Flow Cytometry: OCT4 Antibody (735) [NBP2-15051] - Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [735] dilution: 1:50
View 4 more images

Applications for OCT4 Antibody (735)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OCT4

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Gene Symbol

POU5F1

Additional OCT4 Products

Product Documents for OCT4 Antibody (735)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for OCT4 Antibody (735)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.