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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9B7

Concentration

0.25 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human POU5F1 (NP_002692) produced in E.coli.

Specificity

This antibody is specific for Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1.

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OCT4 Antibody (OTI9B7)

Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923]

Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923]

Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923]

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923]

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY 41186.
Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923]

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923]

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human Ovary tissue using anti-41186 mouse monoclonal antibody.

Applications for OCT4 Antibody (OTI9B7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OCT4

Oct4 is a members of Oct family of transcription factors which specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Entrez Gene IDs

5460 (Human)

Gene Symbol

POU5F1

Additional OCT4 Products

Product Documents for OCT4 Antibody (OTI9B7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OCT4 Antibody (OTI9B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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