OCT4 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2379V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Primate

Predicted:

Feline (93%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all OCT4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Feline (93%), Porcine (93%). Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues. Bovine reactivity reported in scientific literature (PMID: 23054358).

Localization

Nuclear

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for OCT4 Antibody [DyLight 405]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: OCT4

OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Gene Symbol

POU5F1

Additional OCT4 Products

Product Documents for OCT4 Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for OCT4 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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