OCT4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15053

Novus Biologicals, part of Bio-Techne
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NBP2-15053
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (91%), Chimpanzee (100%), Feline (94%), Porcine (93%), Rabbit (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all OCT4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Reactivity Notes

Cat (94%).

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OCT4 Antibody

Western Blot: OCT4 Antibody [NBP2-15053]

Western Blot: OCT4 Antibody [NBP2-15053]

Western Blot: OCT4 Antibody [NBP2-15053] - A. 30 ug human ESC whole cell extract 12% SDS-PAGE Oct4 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody [NBP2-15053]

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody [NBP2-15053]

Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NBP2-15053] - NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053]

Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053]

Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
View 7 more images

Applications for OCT4 Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:500-1:1000

Western Blot

1:5000-1:100000
Application Notes
IHC-FR-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OCT4

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Gene Symbol

POU5F1

UniProt

Q01860

Additional OCT4 Products

Product Documents for OCT4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OCT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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