OGG1 Antibody (8V2Q9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16526

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16526-100ul
NBP3-16526-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8V2Q9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all OGG1 Antibodies »

Product Summary for OGG1 Antibody (8V2Q9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 246-345 of human OGG1 (O15527). VGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OGG1 Antibody (8V2Q9)

Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526]

Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526]

Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526] - Western blot analysis of extracts of various cell lines, using OGG1 Rabbit mAb (NBP3-16526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: OGG1 Antibody (8V2Q9) [NBP3-16526]

Immunohistochemistry-Paraffin: OGG1 Antibody (8V2Q9) [NBP3-16526]

Immunohistochemistry-Paraffin: OGG1 Antibody (8V2Q9) [NBP3-16526] - Immunohistochemistry of paraffin-embedded rat brain using OGG1 Rabbit mAb (NBP3-16526) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526]

Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526]

Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526] - Western blot analysis of extracts of Mouse testis, using OGG1 Rabbit mAb (NBP3-16526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for OGG1 Antibody (8V2Q9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OGG1

Ogg1 encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. 8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. Ogg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine from DNA and cleave the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The Ogg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer. Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases. Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Ogg provides a direct link between DNA damage and toxicity in neurons.

Long Name

8-oxoGuanine DNA Glycosylase

Alternate Names

8-hydroxyguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, AP lyase, DNA-apurinic or apyrimidinic site lyase, HOGG1, MMH, MUTMOGH1HMMH, N-glycosylase/DNA lyase

Gene Symbol

OGG1

Additional OGG1 Products

Product Documents for OGG1 Antibody (8V2Q9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for OGG1 Antibody (8V2Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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