OLFM4 Antibody (PSH01-36)

Western Blot: OLFM4 Antibody (PSH01-36) [NBP3-32677]

Western Blot: OLFM4 Antibody (PSH01-36) [NBP3-32677] - Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (NBP3-32677) at 1/1,000 dilution.

Lane 1: OLFM4 recombinant protein
Lane 2: OLFM4 recombinant protein with immunising peptide

Lysates/proteins at 50 ng/Lane.

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32677) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: OLFM4 Antibody (PSH01-36) [NBP3-32677]

Immunohistochemistry: OLFM4 Antibody (PSH01-36) [NBP3-32677] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-OLFM4 antibody (NBP3-32677) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32677) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.