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Olig2 Antibody (064)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89201

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 064

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, synthetic peptide corresponding to the N-terminus of the Human Olig2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Olig2 Antibody (064)

Western Blot: Olig2 Antibody (064) [NBP2-89201]

Western Blot: Olig2 Antibody (064) [NBP2-89201]

Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa
Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human brain with rabbit monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human glioma with rabbit monoclonal antibody (1:200).

Applications for Olig2 Antibody (064)

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-89201 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Gene Symbol

OLIG2

Additional Olig2 Products

Product Documents for Olig2 Antibody (064)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Olig2 Antibody (064)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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