OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80893
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 kappa Clone # L2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin
Reactivity Notes
Tag specific
Clonality
Monoclonal
Host
Rat
Isotype
IgG1 kappa
Scientific Data Images for OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free
Western Blot: OLLAS Epitope Tag Antibody (L2)Azide and BSA Free [NBP2-80893]
Western Blot: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - OLLAS tagged SFO-p41 protein. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893]
Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody. Image from the standard format of this antibody.Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893]
Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - Different intracellular and transmembrane domains (from Nrx, nSyb, and sdc) were fused to CD19 and expressed into ORNs targeting the DA1 glomerulus using the CD19::Nrx (top), nSyb::CD19 (middle) and CD19::Sdc (bottom), with the R17H02 driver (in green). TApplications for OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free
Application
Recommended Usage
Immunoblotting
reported in scientific literature (PMID 27600537)
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100
Immunohistochemistry-Frozen
1:100
Immunoprecipitation
1:100-1:500
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OLLAS Epitope Tag
Alternate Names
E.coli OmpF Linker and mouse Langerin fusion Sequence, OLLA-2
Additional OLLAS Epitope Tag Products
Product Documents for OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free
Product Specific Notices for OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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