Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Oncostatin M/OSM Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 25-252 of human OSM

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free

Western Blot: Oncostatin M/OSM Antibody (OTI2B6)Azide and BSA Free [NBP2-73146]

Western Blot: Oncostatin M/OSM Antibody (OTI2B6)Azide and BSA Free [NBP2-73146]

Western Blot: Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free [NBP2-73146] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Oncostatin M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Oncostati
Immunohistochemistry: Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free [NBP2-73146]

Immunohistochemistry: Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free [NBP2-73146]

Immunohistochemistry: Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free [NBP2-73146] - Staining of paraffin-embedded lung tissue using anti-Oncostatin M mouse monoclonal antibody.

Applications for Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Oncostatin M/OSM

Oncostatin M is a 252 amino acid protein that is 28 kDa, acts as growth regulator inhibiting the proliferation of a number of tumor cell lines and stimulating the proliferation of AIDS-KS cells; regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells; uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST), and is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. This protein is being studied for its involvement in several diseases and disorders including arterial occlusive disease, peripheral arterial occlusive disease, leukemia, kaposi's sarcoma, abdominal aortic aneurysm, status epilepticus, primary cutaneous amyloidosis, aortic aneurysm, bullous pemphigoid, neonatal jaundice, cutaneous malignant melanoma, plasmacytoma, systemic mastocytosis, mastocytosis, cervical squamous cell carcinoma, systemic lupus erythematosus, allergic rhinitis, squamous cell carcinoma, and lupus erythematosus. The protein has been linked to immune response IL-3 activation and signaling pathway, immune response oncostatin M signaling via JAK-Stat in human cells, development thrombopoetin signaling via JAK-STAT pathway, immune response Oncostatin M signaling via MAPK in human cells, MAPK signaling, endothelin-1 signaling pathway, molecular mechanisms of cancer, antioxidant action of vitamin-C, and Rho Family GTPases pathways where it interacts with IL6ST, TNNT1, LIFR, COL4A4, and COL4A6.

Alternate Names

MGC20461, oncostatin M, oncostatin-M

Gene Symbol

OSM

Additional Oncostatin M/OSM Products

Product Documents for Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Oncostatin M/OSM Antibody (OTI2B6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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