OPA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92221

Novus Biologicals, part of Bio-Techne
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NBP1-92221
NBP1-92221-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all OPA3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RHQLQQRRKEKERRVAREALRGEVGHLGLALEELQAQVQATSTQLALEELRAQLQEVRAHLCLRDP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OPA3 Antibody

Western Blot: OPA3 Antibody [NBP1-92221]

Western Blot: OPA3 Antibody [NBP1-92221]

Western Blot: OPA3 Antibody [NBP1-92221] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: OPA3 Antibody [NBP1-92221]

Immunocytochemistry/ Immunofluorescence: OPA3 Antibody [NBP1-92221]

Immunocytochemistry/Immunofluorescence: OPA3 Antibody [NBP1-92221] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: OPA3 Antibody [NBP1-92221]

Immunohistochemistry-Paraffin: OPA3 Antibody [NBP1-92221]

Immunohistochemistry-Paraffin: OPA3 Antibody [NBP1-92221] - Staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.

Applications for OPA3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OPA3

The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have beenshown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multipletranscript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Alternate Names

FLJ22187, FLJ25932, MGA3Optic atrophy 3 (Iraqi-Jewish 'optic atrophy plus'), MGC75494, optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia), optic atrophy 3 protein

Entrez Gene IDs

8142 (Human)

Gene Symbol

OPA3

UniProt

Q9H6K4 (Human)

Additional OPA3 Products

Product Documents for OPA3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for OPA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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