Orexin R2/HCRTR2 Antibody (1E3)
Novus Biologicals, part of Bio-Techne | Catalog # H00003062-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Knockdown Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1E3
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
HCRTR2 (NP_001517, 1 a.a. ~ 54 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYE
Localization
Isoform 1 and isoform 4: Cell membrane; single pass type I membrane protein. Isoform 2 and isoform 3: Secreted protein.
Specificity
HCRTR2 - hypocretin (orexin) receptor 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Orexin R2/HCRTR2 Antibody (1E3)
![Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]](https://resources.bio-techne.com/images/products/Orexin-R2-HCRTR2-Antibody-1E3-Western-Blot-H00003062-M01-img0006.jpg "Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]")
Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]
Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 expression in transfected 293T cell line by HCRTR2 monoclonal antibody (M01), clone 1E3.Lane 1: HCRTR2 transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.![Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]](https://resources.bio-techne.com/images/products/Orexin-R2-HCRTR2-Antibody-1E3-Western-Blot-H00003062-M01-img0004.jpg "Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]")
Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]
Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 over-expressed 293 cell line, cotransfected with HCRTR2 Validated Chimera RNAi ( Cat # H00003062-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCRTR2 monoclonal antibody (M01), clone 1E3 (Cat # H00003062-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]](https://resources.bio-techne.com/images/products/Orexin-R2-HCRTR2-Antibody-1E3-ELISA-H00003062-M01-img0005.jpg "ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]")
ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]
ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Detection limit for recombinant GST tagged HCRTR2 is approximately 0.03ng/ml as a capture antibody.Applications for Orexin R2/HCRTR2 Antibody (1E3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Reviewed Applications
Read 1 review rated 5 using H00003062-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Orexin R2/HCRTR2
Long Name
Hypocretin Receptor Type 2
Alternate Names
HCRTR2, Orexin Receptor 2, OrexinR2, OX2R
Entrez Gene IDs
3062 (Human)
Gene Symbol
HCRTR2
OMIM
602393 (Human)
UniProt
Additional Orexin R2/HCRTR2 Products
Product Documents for Orexin R2/HCRTR2 Antibody (1E3)
Product Specific Notices for Orexin R2/HCRTR2 Antibody (1E3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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