Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47836

Novus Biologicals, part of Bio-Techne
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NBP2-47836-0.1mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # ODC1/486

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all ODC1 Antibodies »

Product Specifications

Immunogen

Recombinant human Ornithine Decarboxylase protein (Uniprot: P11926)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44734).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free

Western Blot: Ornithine Decarboxylase Antibody (ODC1/486)Azide and BSA Free [NBP2-47836]

Western Blot: Ornithine Decarboxylase Antibody (ODC1/486)Azide and BSA Free [NBP2-47836]

Western Blot: Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free [NBP2-47836] - Western Blot Analysis of PC3 cell lysate usingOrnithine Decarboxylase antibody (ODC1/486).
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free [NBP2-47836]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free [NBP2-47836]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free [NBP2-47836] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/486).
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free [NBP2-47836]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free [NBP2-47836]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free [NBP2-47836] - Human Pancreas stained with ODC1 Monoclonal Antibody (ODC1/486)
View 2 more images

Applications for Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ODC1

Ornithine Decarboxylase encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.

Long Name

Ornithine Decarboxylase 1

Alternate Names

ODC

Gene Symbol

ODC1

Additional ODC1 Products

Product Documents for Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ornithine Decarboxylase Antibody (ODC1/486) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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