ORP150/HSP12A Antibody (6F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00010525-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ORP150/HSP12A Antibodies »

Product Specifications

Immunogen

HYOU1 (NP_006380, 901 a.a. ~ 999 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVQYLLNKAKFTKPRPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPLELGGPGAEPEQKEQSTGQKRPLKNDEL

Reactivity Notes

Other species not tested.

Specificity

HYOU1 - hypoxia up-regulated 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ORP150/HSP12A Antibody (6F7)

Western Blot: ORP150/HSP12A Antibody (6F7) [H00010525-M01]

Western Blot: ORP150/HSP12A Antibody (6F7) [H00010525-M01]

Western Blot: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - HYOU1 monoclonal antibody (M01), clone 6F7 Analysis of HYOU1 expression in MCF-7.
Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6F7) [H00010525-M01]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6F7) [H00010525-M01]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - Analysis of monoclonal antibody to HYOU1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.
ELISA: ORP150/HSP12A Antibody (6F7) [H00010525-M01]

ELISA: ORP150/HSP12A Antibody (6F7) [H00010525-M01]

ELISA: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - Detection limit for recombinant GST tagged HYOU1 is approximately 0.03ng/ml as a capture antibody.

Applications for ORP150/HSP12A Antibody (6F7)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ORP150/HSP12A

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Entrez Gene IDs

10525 (Human)

Gene Symbol

HYOU1

OMIM

601746 (Human)

UniProt

NP_006380 (Human)

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody (6F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ORP150/HSP12A Antibody (6F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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