ORP150/HSP12A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14113

Novus Biologicals, part of Bio-Techne
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NBP2-14113
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Key Product Details

Species Reactivity

Validated:

Human, Chicken

Cited:

Avian - Chicken

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ORP150/HSP12A Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: VLQLINDNTATALSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLRERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRV

Reactivity Notes

Chicken reactivity reported in literature (PMID: 30298003)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ORP150/HSP12A Antibody

Western Blot: ORP150/HSP12A Antibody [NBP2-14113]

Western Blot: ORP150/HSP12A Antibody [NBP2-14113]

Western Blot: ORP150/HSP12A Antibody [NBP2-14113] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HELA
Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-14113]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-14113]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-14113] - Staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.
Simple Western: ORP150/HSP12A Antibody [NBP2-14113]

Simple Western: ORP150/HSP12A Antibody [NBP2-14113]

Simple Western: ORP150/HSP12A Antibody [NBP2-14113] - Simple Western lane view shows a specific band for ORP150/HSP12A in 0.2 mg/ml of HEL lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
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Applications for ORP150/HSP12A Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ORP150/HSP12A

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Gene Symbol

HYOU1

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ORP150/HSP12A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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