Osteocalcin Antibody (2D4)
Novus Biologicals, part of Bio-Techne | Catalog # H00000632-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Lambda Clone # 2D4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BGLAP (NP_954642, 52 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV
Specificity
BGLAP - bone gamma-carboxyglutamate (gla) protein (osteocalcin)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Lambda
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Osteocalcin Antibody (2D4)
![Western Blot: Osteocalcin Antibody (2D4) [H00000632-M01]](https://resources.bio-techne.com/images/products/Osteocalcin-Antibody-2D4-Western-Blot-H00000632-M01-img0007.jpg "Western Blot: Osteocalcin Antibody (2D4) [H00000632-M01]")
Western Blot: Osteocalcin Antibody (2D4) [H00000632-M01]
Western Blot: Osteocalcin Antibody (2D4) [H00000632-M01] - Analysis of BGLAP expression in transfected 293T cell line by BGLAP monoclonal antibody (M01), clone 2D4.Lane 1: BGLAP transfected lysate(11 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2D4) [H00000632-M01]](https://resources.bio-techne.com/images/products/Osteocalcin-Antibody-2D4-Immunocytochemistry-Immunofluorescence-H00000632-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2D4) [H00000632-M01]")
Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2D4) [H00000632-M01]
Immunocytochemistry/Immunofluorescence: Osteocalcin Antibody (2D4) [H00000632-M01] - Analysis of monoclonal antibody to BGLAP on HeLa cell . Antibody concentration 10 ug/ml.![ELISA: Osteocalcin Antibody (2D4) [H00000632-M01]](https://resources.bio-techne.com/images/products/Osteocalcin-Antibody-2D4-ELISA-H00000632-M01-img0006.jpg "ELISA: Osteocalcin Antibody (2D4) [H00000632-M01]")
ELISA: Osteocalcin Antibody (2D4) [H00000632-M01]
ELISA: Osteocalcin Antibody (2D4) [H00000632-M01] - Detection limit for recombinant GST tagged BGLAP is approximately 0.3ng/ml as a capture antibody.Applications for Osteocalcin Antibody (2D4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:500
Application Notes
Use in IHC reported in scientific literature (PMID:34948393).Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF..
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Osteocalcin
Long Name
Bone gamma-Carboxyglutamate [gla] Protein
Alternate Names
BGLAP, BGP, OCN
Entrez Gene IDs
632 (Human)
Gene Symbol
BGLAP
OMIM
112260 (Human)
UniProt
Additional Osteocalcin Products
Product Documents for Osteocalcin Antibody (2D4)
Product Specific Notices for Osteocalcin Antibody (2D4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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