Osteocalcin Antibody (2T1J7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16081
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2T1J7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein of human Osteocalcin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Osteocalcin Antibody (2T1J7)
![Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]](https://resources.bio-techne.com/images/products/Osteocalcin-Antibody-2T1J7-Western-Blot-NBP3-16081-img0001.jpg "Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]")
Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]
Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of extracts of various cell lines, using Osteocalcin antibody (NBP3-16081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.![Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]](https://resources.bio-techne.com/images/products/Osteocalcin-Antibody-2T1J7-Western-Blot-NBP3-16081-img0002.jpg "Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]")
Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]
Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of extracts of various cell lines, using Osteocalcin antibody (NBP3-16081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
ICC/IF: Osteocalcin Antibody (2T1J7) [NBP3-16081] -
ICC/IF: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of NIH/3T3 cells using Osteocalcin (BGLAP) Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for Osteocalcin Antibody (2T1J7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Osteocalcin
Long Name
Bone gamma-Carboxyglutamate [gla] Protein
Alternate Names
BGLAP, BGP, OCN
Gene Symbol
BGLAP
Additional Osteocalcin Products
Product Documents for Osteocalcin Antibody (2T1J7)
Product Specific Notices for Osteocalcin Antibody (2T1J7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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