Osteocalcin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13133
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (91%), Chimpanzee (97%), Porcine (91%), Rhesus Macaque (95%), Sheep (91%). Backed by our 100% Guarantee.
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the Center region of human Osteocalcin. The exact sequence is proprietary.
Reactivity Notes
Chicken (81%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Osteocalcin Antibody
Western Blot: Osteocalcin Antibody [NBP3-13133]
Western Blot: Osteocalcin Antibody [NBP3-13133] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: Osteocalcin Antibody [NBP3-13133]
Western Blot: Osteocalcin Antibody [NBP3-13133] - Recombinant Osteocalcin were separated by 10% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.ELISA: Osteocalcin Antibody [NBP3-13133]
ELISA: Osteocalcin Antibody [NBP3-13133] - Indirect ELISA analysis was performed by coating plate with 50 uL of recombinant full-length Osteocalcin protein at concentrations ranging from 0.938 ng/mL to 60 ng/mL. The coated protein is detected with Osteocalcin antibody (NBP3-13133) at 1 ug/mL.Applications for Osteocalcin Antibody
Application
Recommended Usage
ELISA
1:1000-1:10000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Osteocalcin
Long Name
Bone gamma-Carboxyglutamate [gla] Protein
Alternate Names
BGLAP, BGP, OCN
Gene Symbol
BGLAP
Additional Osteocalcin Products
Product Specific Notices for Osteocalcin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...