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Osteocalcin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13133

Novus Biologicals, part of Bio-Techne
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NBP3-13133

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (91%), Chimpanzee (97%), Porcine (91%), Rhesus Macaque (95%), Sheep (91%). Backed by our 100% Guarantee.

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the Center region of human Osteocalcin. The exact sequence is proprietary.

Reactivity Notes

Chicken (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Osteocalcin Antibody

Western Blot: Osteocalcin Antibody [NBP3-13133]

Western Blot: Osteocalcin Antibody [NBP3-13133]

Western Blot: Osteocalcin Antibody [NBP3-13133] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Osteocalcin Antibody [NBP3-13133]

Western Blot: Osteocalcin Antibody [NBP3-13133]

Western Blot: Osteocalcin Antibody [NBP3-13133] - Recombinant Osteocalcin were separated by 10% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
ELISA: Osteocalcin Antibody [NBP3-13133]

ELISA: Osteocalcin Antibody [NBP3-13133]

ELISA: Osteocalcin Antibody [NBP3-13133] - Indirect ELISA analysis was performed by coating plate with 50 uL of recombinant full-length Osteocalcin protein at concentrations ranging from 0.938 ng/mL to 60 ng/mL. The coated protein is detected with Osteocalcin antibody (NBP3-13133) at 1 ug/mL.

Applications for Osteocalcin Antibody

Application
Recommended Usage

ELISA

1:1000-1:10000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteocalcin

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Long Name

Bone gamma-Carboxyglutamate [gla] Protein

Alternate Names

BGLAP, BGP, OCN

Gene Symbol

BGLAP

Additional Osteocalcin Products

Product Documents for Osteocalcin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Osteocalcin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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