Osteopontin/OPN Antibody (1B20) [DyLight 488]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-89062G

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B20

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Osteopontin/OPN Antibodies »

Product Summary for Osteopontin/OPN Antibody (1B20) [DyLight 488]

Immunogen

Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].

Reactivity Notes

Human, Rat and Rabbit. Rabbit reactivity reported in scientific literature (PMID:33001866). Use in Mouse reported in scientific literature (PMID:33580126).

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Osteopontin/OPN Antibody (1B20) [DyLight 488]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Osteopontin/OPN

The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (1B20) [DyLight 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Osteopontin/OPN Antibody (1B20) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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