Osteopontin/OPN Antibody (1B20) [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-89062H

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB110-89062AF350

Alexa Fluor 405

NB110-89062AF405

Alexa Fluor 488

NB110-89062AF488

Alexa Fluor 532

NB110-89062AF532

Alexa Fluor 594

NB110-89062AF594

Alexa Fluor 647

NB110-89062AF647

Alexa Fluor 700

NB110-89062AF700

Alexa Fluor 750

NB110-89062AF750

Biotin

NB110-89062B

CoraFluor 1

NB110-89062CL1

DyLight 350

NB110-89062UV

DyLight 405

NB110-89062V

DyLight 488

NB110-89062G

DyLight 550

NB110-89062R

DyLight 594

NB110-89062DL594

DyLight 650

NB110-89062C

DyLight 680

NB110-89062FR

DyLight 755

NB110-89062IR

FITC

NB110-89062F

Janelia Fluor 525

NB110-89062JF525

Janelia Fluor 549

NB110-89062JF549

Janelia Fluor 646

NB110-89062JF646

Janelia Fluor 669

NB110-89062JF669

mFluor Violet 450 SE

NB110-89062MFV450

mFluor Violet 500 SE

NB110-89062MFV500

mFluor Violet 610 SE

NB110-89062MFV610

Forumulation

Catalog #

BSA Free

NB110-89062

Azide and BSA Free

NBP2-80895

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

HRP

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1B20

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Osteopontin/OPN Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].

Reactivity Notes

Human, Rat and Rabbit. Rabbit reactivity reported in scientific literature (PMID:33001866). Use in Mouse reported in scientific literature (PMID:33580126).

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Osteopontin/OPN Antibody (1B20) [HRP]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Osteopontin/OPN

The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (1B20) [HRP]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Osteopontin/OPN Antibody (1B20) [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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