Osteopontin/OPN Antibody (CL10686)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07980

Novus Biologicals, part of Bio-Techne
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NBP3-07980-100ul
NBP3-07980-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10686

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Osteopontin/OPN Antibodies »

Product Summary for Osteopontin/OPN Antibody (CL10686)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDAT

Epitope

GRGDSVVYGLRSKSK

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Osteopontin/OPN Antibody (CL10686)

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Analysis in human cerebral cortex and tonsil tissues using NBP3-07980 antibody. Corresponding Osteopontin/OPN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human cerebellum shows moderate cytoplasmic positivity in microglia.
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Applications for Osteopontin/OPN Antibody (CL10686)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (CL10686)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Osteopontin/OPN Antibody (CL10686)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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