Osteopontin/OPN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1043

Novus Biologicals, part of Bio-Techne
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NB600-1043
NB600-1043-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Osteopontin/OPN Antibodies »

Product Specifications

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human Osteopontin/OPN protein, conjugated to KLH using maleimide. (Uniprot: P10451)

Localization

Secreted

Specificity

Osteopontin/OPN is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Osteopontin/OPN Antibody

Western Blot: Osteopontin/OPN Antibody [NB600-1043]

Western Blot: Osteopontin/OPN Antibody [NB600-1043]

Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Western Blot of Osteopontin/OPN/OPN antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin/OPN [250ng]. Lane 3: MMP-cleaved Osteopontin/OPN. Primary Antibody: Anti-Osteopontin/OPN at 1:1000 ovenight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.
Immunohistochemistry: Osteopontin/OPN Antibody [NB600-1043]

Immunohistochemistry: Osteopontin/OPN Antibody [NB600-1043]

Immunohistochemistry: Osteopontin/OPN Antibody [NB600-1043] - Osteopontin/OPN/OPN antibody was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin/OPN is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin/OPN is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Osteopontin/OPN Antibody

Osteopontin/OPN Antibody

Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.

Applications for Osteopontin/OPN Antibody

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunohistochemistry

1:100-1:300

Immunoprecipitation

1:100

Western Blot

1:500-1:2000
Application Notes
This product has been tested for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections) and ELISA. The antibody exclusively recognizes C-terminal fragments of both thrombin and MMP-cleaved OPN. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), and 32 kDa for the MMP-cleaved C-fragment, but the 40 kDa N-terminal fragment is not recognized. A 1:1000 dilution will detect strongly approximately 250 ng of OPN protein on a blot. A 1:100-1:300 dilution used for IHC on human breast tumor tissues. No pretreatment is required for IHC when formalin-fixed paraffin-embedded tissue is stained. Specific conditions for reactivity and signal detection should be optimized by the end user.

Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 29506220).

Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 26963743).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Entrez Gene IDs

6696 (Human); 25353 (Rat)

Gene Symbol

SPP1

UniProt

p10451 (Human)

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Osteopontin/OPN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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