Osteopontin/OPN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89952

Novus Biologicals, part of Bio-Techne
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NBP1-89952
NBP1-89952-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Osteopontin/OPN Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Osteopontin/OPN Antibody

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human placenta and liver tissues. Corresponding SPP1 RNA-seq data are presented for the same tissues.
Western Blot: Osteopontin/OPN Antibody [NBP1-89952]

Western Blot: Osteopontin/OPN Antibody [NBP1-89952]

Western Blot: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952]

Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952]

Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus. Antibody staining is shown in green.
View 4 more images

Applications for Osteopontin/OPN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Osteopontin/OPN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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