Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56505

Novus Biologicals, part of Bio-Techne
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NB100-56505
NB100-56505SS
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Azide Free
NBP2-33211
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human, Mouse, Rat, Guinea Pig, Rabbit

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 98A1071

Format

BSA Free

Concentration

1.0 mg/ml

View all Osteoprotegerin/TNFRSF11B Antibodies »

Product Specifications

Immunogen

A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human Osteoprotegerin was used as immunogen for this antibody.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 31658685).

Specificity

The Osteoprotegerin antibody, clone 98A1071, detects all forms of Osteoprotegerin including monomeric, dimeric and Osteoprotegerin bound to RANKL (Vandooren, 2008) as well as Osteoprotegerin bound to TRAIL (Sandra, 2006).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free [NB100-56505]

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free [NB100-56505]

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Human liver using 1:200 dilution of anti-Osteoprotegerin/TNFRSF11B antibody (clone 98A1071). The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Osteoprotegerin antibody generated a diffused but weak cytoplasmic staining with a very specific and strong punctate signal at the periphery of the cells.
Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071)BSA Free [NB100-56505]

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071)BSA Free [NB100-56505]

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Osteoprotegerin in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free [NB100-56505]

Immunocytochemistry/ Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free [NB100-56505]

Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Osteoprotegerin (98A1071) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Applications for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 15935726)

Flow Cytometry

0.2-1 ug/1 million cells

Immunocytochemistry/ Immunofluorescence

1:20-1:1000. Use reported in scientific literature (Sandra (2006))

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:20-1:1000. Use reported in scientific literature (Boumans (2012))

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:20-1:1000. Use reported in scientific literature (Sandra (2006))

Western Blot

1 ug/ml
Application Notes
This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor (TNFR) superfamily. As the name implies, it protects bone. Unlike other TNFRs it lacks a transmembrane domain and lacks any apparent cell associated signals. OPG consists of 401 amino acid with molecular mass of approximately 55 kD as a monomer and 110 kD as a disulfide-linked dimer. High levels of OPG mRNA has been detected in lung, heart, kidney, and placenta1. Recombinant OPG blocks osteoclastogenesis in vitro and increases bone density in vivo. Targeted deletion of OPG in mice results in severe early-onset of osteoporosity. These mice also exhibit increase in vascular calcification in the aorta and renal arteries suggesting that it may also play a role in degenerative arterial disease.

Alternate Names

OCIF, TNFRSF11B

Entrez Gene IDs

690 (Human)

Gene Symbol

TNFRSF11B

UniProt

O00300

Additional Osteoprotegerin/TNFRSF11B Products

Product Documents for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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