Otubain-1 Antibody (GT611)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13627
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT611
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse Otubain-1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Otubain-1 Antibody (GT611)
![Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627]](https://resources.bio-techne.com/images/products/Otubain-1-Antibody-GT611-Western-Blot-NBP3-13627-img0001.jpg "Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627]")
Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627]
Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Otubain-1 Antibody (GT611) [NBP3-13627]](https://resources.bio-techne.com/images/products/Otubain-1-Antibody-GT611-Immunocytochemistry-Immunofluorescence-NBP3-13627-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Otubain-1 Antibody (GT611) [NBP3-13627]")
Immunocytochemistry/ Immunofluorescence: Otubain-1 Antibody (GT611) [NBP3-13627]
Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody (GT611) [NBP3-13627] - Otubain-1 antibody [GT611] detects Otubain-1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Otubain-1 stained by Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.Applications for Otubain-1 Antibody (GT611)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Otubain-1
Long Name
OTU Domain, Ubiquitin Aldehyde Binding 1
Alternate Names
OTB1, OTU1, OTUB1, Otubain1
Gene Symbol
OTUB1
Additional Otubain-1 Products
Product Documents for Otubain-1 Antibody (GT611)
Product Specific Notices for Otubain-1 Antibody (GT611)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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