Otubain-1: Lysates

Otubain-1, also known as OTUB1 or Ubiquitin Thioesterase, is a member of the conserved ovarian tumor (OTU) protein family. It is a 271 amino acid (aa) polypeptide with a predicted molecular weight of 32 kDa. Otubain-1 is 99% identical at the aa level to its mouse and rat orthologs. It is a deubiquitinating enzyme (DUB) that appears to exhibit specificity for K48 Ubiquitin linkages. However, Otubain-1 may also affect K63 linkages independent of DUB activity. For instance, Otubain-1 can directly bind to and suppress UBC13, a Ubiquitin Ligase (E2) that catalyzes the formation K63-linked Ubiquitin chains in response to DNA damage.