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Otubain-2 Antibody (OTI11B3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03223

Novus Biologicals, part of Bio-Techne
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NBP2-03223
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11B3

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human OTUB2 (NP_075601) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Otubain-2 Antibody (OTI11B3)

Western Blot: Otubain-2 Antibody (OTI11B3) [NBP2-03223]

Western Blot: Otubain-2 Antibody (OTI11B3) [NBP2-03223]

Western Blot: Otubain-2 Antibody (11B3) [NBP2-03223] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Otubain-2 monoclonal antibody.
Flow Cytometry: Otubain-2 Antibody (OTI11B3) [NBP2-03223]

Flow Cytometry: Otubain-2 Antibody (OTI11B3) [NBP2-03223]

Flow Cytometry: Otubain-2 Antibody (11B3) [NBP2-03223] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Otubain-2 Antibody, and then analyzed by flow cytometry.
Western Blot: Otubain-2 Antibody (OTI11B3) [NBP2-03223]

Western Blot: Otubain-2 Antibody (OTI11B3) [NBP2-03223]

Western Blot: Otubain-2 Antibody (11B3) [NBP2-03223] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2.

Applications for Otubain-2 Antibody (OTI11B3)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Otubain-2

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.[supplied by OMIM].

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 2

Alternate Names

C14orf137, OTU2, OTUB2, Otubain2

Entrez Gene IDs

78990 (Human)

Gene Symbol

OTUB2

Additional Otubain-2 Products

Product Documents for Otubain-2 Antibody (OTI11B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Otubain-2 Antibody (OTI11B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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