Otubain-2 Antibody (OTI11B3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73172

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11B3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Otubain-2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human OTUB2 (NP_075601) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Otubain-2 Antibody (OTI11B3) - Azide and BSA Free

Western Blot: Otubain-2 Antibody (OTI11B3)Azide and BSA Free [NBP2-73172]

Western Blot: Otubain-2 Antibody (OTI11B3)Azide and BSA Free [NBP2-73172]

Western Blot: Otubain-2 Antibody (OTI11B3) - Azide and BSA Free [NBP2-73172] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2.
Flow Cytometry: Otubain-2 Antibody (OTI11B3) - Azide and BSA Free [NBP2-73172]

Flow Cytometry: Otubain-2 Antibody (OTI11B3) - Azide and BSA Free [NBP2-73172]

Flow Cytometry: Otubain-2 Antibody (OTI11B3) - Azide and BSA Free [NBP2-73172] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Otubain-2 Antibody, and then analyzed by flow cytometry.
Western Blot: Otubain-2 Antibody (OTI11B3)Azide and BSA Free [NBP2-73172]

Western Blot: Otubain-2 Antibody (OTI11B3)Azide and BSA Free [NBP2-73172]

Western Blot: Otubain-2 Antibody (OTI11B3) - Azide and BSA Free [NBP2-73172] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Otubain-2 monoclonal antibody.

Applications for Otubain-2 Antibody (OTI11B3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Otubain-2

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 2

Alternate Names

C14orf137, OTU2, OTUB2, Otubain2

Gene Symbol

OTUB2

Additional Otubain-2 Products

Product Documents for Otubain-2 Antibody (OTI11B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Otubain-2 Antibody (OTI11B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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