Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all OTUD4 Antibodies »

Product Summary for OTUD4 Antibody

Immunogen

This antibody maps to a region between residue 875 to 925 of human OTU Domain Dontaining 4 using the numbering given in entry XP_005263137.1 (GeneID 54726).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OTUD4 Antibody

Western Blot: OTUD4 Antibody [NBP2-37689]

Western Blot: OTUD4 Antibody [NBP2-37689]

Western Blot: OTUD4 Antibody [NBP2-37689] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-OTUD4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: OTUD4 Antibody [NBP2-37689]

Immunoprecipitation: OTUD4 Antibody [NBP2-37689]

Immunoprecipitation: OTUD4 Antibody [NBP2-37689] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-OTUD4 antibody used for IP at 6 ug per reaction. OTUD4 was also immunoprecipitated by rabbit anti-OTUD4 antibody A304-606A. For blotting immunoprecipitated OTUD4, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for OTUD4 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: OTUD4

OTUD4 is a gene that codes for a protein with four isoforms, with lengths of 1113, 210, 145, and 1048 amino acids and weights of approximately 124, 24, 17, and 117 kDa respectively. OTUD4 has been shown to have interactions with AGO2, ATG12, BAG5, DSG1, and FXR2.

Alternate Names

DKFZp434I0721, HIV-1 induced protein HIN-1, HIV-1-induced protein HIN-1, HSHIN1, KIAA1046HIN1DUBA6, OTU domain containing 4, OTU domain-containing protein 4

Gene Symbol

OTUD4

UniProt

XP_005263137.1 (Human)

Additional OTUD4 Products

Product Documents for OTUD4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OTUD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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