Otx2 Antibody (1H12C4B5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37597

Novus Biologicals, part of Bio-Techne
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NBP2-37597
NBP2-37597-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H12C4B5

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Otx2 Antibodies »

Product Summary for Otx2 Antibody (1H12C4B5) - BSA Free

Immunogen

Purified recombinant fragment of human OTX2 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Otx2 Antibody (1H12C4B5) - BSA Free

Western Blot: Otx2 Antibody (1H12C4B5)BSA Free [NBP2-37597]

Western Blot: Otx2 Antibody (1H12C4B5)BSA Free [NBP2-37597]

Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - ARPE-19 monolayer on glass substrate. Fixative 4% PFA Blocking Goat Serum Dilution 1:200. Incubation 1h @RT. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.
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Applications for Otx2 Antibody (1H12C4B5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-37597 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

Additional Otx2 Products

Product Documents for Otx2 Antibody (1H12C4B5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Otx2 Antibody (1H12C4B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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