Otx2 Antibody (6I3J0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16384

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16384-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6I3J0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Otx2 Antibodies »

Product Summary for Otx2 Antibody (6I3J0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 190-289 of human OTX2 (P32243). AGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Otx2 Antibody (6I3J0)

Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384]

Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384]

Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384] - Western blot analysis of extracts of various cell lines, using Otx2 Rabbit mAb (NBP3-16384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384]

Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384]

Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384] - Western blot analysis of extracts of various cell lines, using Otx2 Rabbit mAb (NBP3-16384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for Otx2 Antibody (6I3J0)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

Additional Otx2 Products

Product Documents for Otx2 Antibody (6I3J0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Otx2 Antibody (6I3J0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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