Otx2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13176

Novus Biologicals, part of Bio-Techne
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NBP3-13176
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Otx2 Antibodies »

Product Summary for Otx2 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Otx2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Otx2 Antibody

Western Blot: Otx2 Antibody [NBP3-13176]

Western Blot: Otx2 Antibody [NBP3-13176]

Western Blot: Otx2 Antibody [NBP3-13176] - Whole cell extract (37.5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otx2 antibody (NBP3-13176) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: Otx2 Antibody [NBP3-13176]

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP3-13176]

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP3-13176] - Otx2 antibody detects Otx2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Otx2 stained by Otx2 antibody (NBP3-13176) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Frozen: Otx2 Antibody [NBP3-13176]

Immunohistochemistry-Frozen: Otx2 Antibody [NBP3-13176]

Immunohistochemistry-Frozen: Otx2 Antibody [NBP3-13176] - Otx2 antibody detects Otx2 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Otx2 stained by Otx2 antibody (NBP3-13176) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for Otx2 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

Additional Otx2 Products

Product Documents for Otx2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Otx2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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