OX40/TNFRSF4 Antibody (BLR042F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76421

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76421
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR042F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all OX40/TNFRSF4 Antibodies »

Product Specifications

Immunogen

Recombinant protein representing the extracellular domain of human OX40/CD134

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OX40/TNFRSF4 Antibody (BLR042F)

Western Blot: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421]

Western Blot: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421]

Western Blot: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Detection of human OX40/CD134 by WB of HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 lysate. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal [BLR042F] (NBP2-76421). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/ Immunofluorescence: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421]

Immunocytochemistry/ Immunofluorescence: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421]

Immunocytochemistry/Immunofluorescence: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Detection of human OX40/CD134 (red) in FFPE breast carcinoma by IHC-IF. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal [BLR042F] (NBP2-76421). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421]

Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421]

Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Section of MJ cells. Antibody: Rabbit anti-OX40/CD134 recombinantmonoclonal antibody [BLR042F] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.
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Applications for OX40/TNFRSF4 Antibody (BLR042F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/1mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: OX40/TNFRSF4

OX40/CD134/TNFSF4 is a member of the TNF-receptor superfamily that has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested OX40/CD134 plays a role in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.

Alternate Names

ACT-135, ACT35 antigen, ACT35ATC35 antigen, CD134, CD134 antigen, Ly-70, OX40 cell surface antigen, OX40 homologue, OX40L receptor, OX40lymphoid activation antigene ACT35, TAX transcriptionally-activated glycoprotein 1 receptor, tax-transcriptionally activated glycoprotein 1 receptor, TNFRSF4, tumor necrosis factor receptor superfamily member 4, tumor necrosis factor receptor superfamily, member 4, Txgp1, TXGP1L, TXGP1LOX40 antigen

Gene Symbol

TNFRSF4

Additional OX40/TNFRSF4 Products

Product Documents for OX40/TNFRSF4 Antibody (BLR042F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OX40/TNFRSF4 Antibody (BLR042F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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