OXPAT Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60509

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Bovine

Cited:

Human, Mouse, Porcine

Predicted:

Sheep (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all OXPAT Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a region of the human OXPAT protein, within residues 300-400. [UniProt# Q00G26]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29094660). Immunogen displays the following percentage of sequence identity for non-tested species: sheep (94%), rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OXPAT Antibody - BSA Free

Western Blot: OXPAT AntibodyBSA Free [NB110-60509]

Western Blot: OXPAT AntibodyBSA Free [NB110-60509]

Western Blot: OXPAT Antibody [NB110-60509] - Detection of OXPAT in human (lane 1) and mouse (lane 2) heart lysate.
Immunohistochemistry: OXPAT Antibody - BSA Free [NB110-60509]

Immunohistochemistry: OXPAT Antibody - BSA Free [NB110-60509]

Immunohistochemistry: OXPAT Antibody [NB110-60509] - Staining of OXPAT in paraffin embedded mouse fat.
Simple Western: OXPAT AntibodyBSA Free [NB110-60509]

Simple Western: OXPAT AntibodyBSA Free [NB110-60509]

Simple Western: OXPAT Antibody [NB110-60509] - Simple Western lane view shows a specific band for OXPAT in 0.5 mg/ml of Human Heart (left) and Mouse Heart (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for OXPAT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 26096040)

Immunohistochemistry

1.0-5.0 ug/ml

Immunohistochemistry-Frozen

reported in scientific literature (PMID 24648545)

Immunohistochemistry-Paraffin

1.0-5.0 ug/ml

Simple Western

10 ug/ml

Western Blot

0.5-2.0 ug/ml
Application Notes
In WB a band can be seen at approx. 50 kDa, representing the OXPAT protein.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OXPAT

OXPAT (PLIN5/Perilipin 5) is a novel PAT-1 (Perilipin, ADRP and TIP47) that binds to lipid droplets (LDs) and protects them from lipolytic degradation. The tissue distribution of PAT-1 proteins differs considerably, however, all of them are involved in accumulation and metabolism of lipids in LDs. OXPAT covers LDs in the cytosol and its expression is highest in tissues with a high fat-oxidative capacity, such as heart, oxidative (type I) skeletal muscles and brown adipose tissue wherein the OXPAT contents parallel the LD content. OXPAT overexpression in OP-9 and COS-7 cells increased cellular triglyceride storage in parallel with increases in fatty acid oxidation and induction of gene expression of mitochondrial enzymes involved in oxidative metabolism. OXPAT has the potential of modulating the interaction of the major triacylglycerol lipase ATGL with LD as well as with its co-factor CGI-58 in a well control manner to regulate LD lipolysis. OXPAT expression is up-regulated by PPARalpha, and this regulation appears to be part of an expression program that shifts the metabolism of cells from fatty acid storage to oxidation.

Alternate Names

lipid storage droplet protein 5, LSDA5, LSDP5, MLDP, OXPAT, perilipin 5, perilipin-5

Gene Symbol

PLIN5

Additional OXPAT Products

Product Documents for OXPAT Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OXPAT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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