P-Cadherin Antibody (OTI2D5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73267

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all P-Cadherin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CDH3(NP_001784) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

88.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for P-Cadherin Antibody (OTI2D5) - Azide and BSA Free

Western Blot: P-Cadherin Antibody (OTI2D5)Azide and BSA Free [NBP2-73267]

Western Blot: P-Cadherin Antibody (OTI2D5)Azide and BSA Free [NBP2-73267]

Western Blot: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY P-Cadherin.
Immunocytochemistry/ Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267]

Immunocytochemistry/ Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267]

Immunocytochemistry/Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY P-Cadherin.
Immunocytochemistry/ Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267]

Immunocytochemistry/ Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267]

Immunocytochemistry/Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267] - Analysis of HeLa cells.

Applications for P-Cadherin Antibody (OTI2D5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: P-Cadherin

The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. R-cadherin (for retinal-cadherin, also designated cadherin-4), which was first identified in the retina of chicken, has been shown to be involved in the development of striated muscle and potentially epithelia in addition to its involvement in retinal development.

Long Name

Placental Cadherin

Alternate Names

CAD3, Cadherin-3, CDH3, CDHP, HJMD, PCAD, PCadherin

Gene Symbol

CDH3

Additional P-Cadherin Products

Product Documents for P-Cadherin Antibody (OTI2D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for P-Cadherin Antibody (OTI2D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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